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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
10/11/2023 |
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Actualizado : |
10/11/2023 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
BORGES, M.A.; CURCIO, B.R.; GASTAL, G.D.A.; GHENO, L.; JUNIOR, A.S.V.; CORCINI, C.D.; NOGUEIRA, C.E.W.; AGUIAR, F.L.N.; GASTAL, E.L. |
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Afiliación : |
MORGANA A. BORGES, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; BRUNA R. CURCIO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; GUSTAVO DESIRE ANTUNES GASTAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIZA GHENO, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; ANTONIO S. VARELA JUNIOR, Department of Comparative Animal Reproduction, Institute of Biological Sciences, Federal University of Rio Grande, RS, Rio Grande, Brazil; CARINE D. CORCINI, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; CARLOS E.W. NOGUEIRA, Department of Veterinary Clinics, College of Veterinary Medicine, Federal University of Pelotas, RS, Pelotas, Brazil; FRANCISCO L.N. AGUIAR, Department of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraiba, PB, Sousa, Brazil; EDUARDO L. GASTAL, Animal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, IL, United States. |
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Título : |
Ethanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Reproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814 |
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ISSN : |
1642-431X |
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DOI : |
10.1016/j.repbio.2023.100814 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. -- |
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Contenido : |
ABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn MenosABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more ... Presentar Todo |
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Palabras claves : |
Fixative solution; Mare; Ovarian tissue quality; Preantral follicle morphometry. |
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Asunto categoría : |
L50 Fisiología y bioquímica animal |
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Marc : |
LEADER 03765naa a2200301 a 4500
001 1064365
005 2023-11-10
008 2023 bl uuuu u00u1 u #d
022 $a1642-431X
024 7 $a10.1016/j.repbio.2023.100814$2DOI
100 1 $aBORGES, M.A.
245 $aEthanol, Carnoy, and paraformaldehyde as fixative solutions for histological evaluation of preantral follicles in equine ovarian tissue.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 28 February 2023; Received in revised form 24 September 2023; Accepted 27 September 2023; Available online 25 October 2023. -- Correspondence author: Gastal, E.L.; Animal Science, School of Agricultural Sciences, Southern Illinois University, 1205 Lincoln Drive, MC 4417, Carbondale, IL, United States; email:egastal@siu.edu -- FUNDING: This research was supported by grants from the Brazilian Criollo Horse Breeder Association (ABCCC) and Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS; grant no. 21/2551-0002239-6). A.S. Varela Junior, C.D. Corcini, and B.R. Curcio had research productivity fellowships from the National Council for Scientific and Technological Development (CNPq). M.A. Borges and L. Gheno received DS and MS scholarships, respectively, from the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazil. --
520 $aABSTRACT.- The most adequate fixative solution for equine ovarian tissue is still to be determined as a tool to evaluate the improvement of methodological studies in assisted reproductive techniques and fertility preservation. This study aimed to evaluate a short-time ethanol 70% (ST-EtOH, 45 min) exposure as an alternative fixative compared with two classically fixatives [Carnoy's (CAR) solution and paraformaldehyde 4% (PFA)] at different fixation times (6 h, 12 h). The end points evaluated were morphology and classes of preantral follicles, follicular and stromal cell densities, and follicular and oocyte nuclear diameters in equine ovarian tissue. Ovaries (n = 6) from ovariectomized young mares were fragmented (3 × 3 × 1 mm; 20 fragments/ovary) and fixed in the tested treatments. Overall, a total of 11,661 preantral follicles were evaluated in 1444 histological slides. The ST-EtOH similarly preserved the preantral follicle morphometry and stromal cell density compared to the PFA fixative, regardless of the exposure time. Nonetheless, the CAR fixative solution had the greatest percentage of normal preantral follicles and the highest stromal cell density among all treatments. In conclusion, Carnoy's solution must be preferred compared with ST-EtOH and PFA fixatives for studies concerning the cellular morphology of equine ovarian tissue. Moreover, ST-EtOH fixative is a good alternative for equine ovarian tissue when a quick histological evaluation is required instead of more time-consuming and expensive techniques. Additional studies concerning the impact of different fixatives on the ultrastructure of cellular populations and their compatibility with IHC and molecular techniques in equine ovarian tissue are warranted. © 2023 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn
653 $aFixative solution
653 $aMare
653 $aOvarian tissue quality
653 $aPreantral follicle morphometry
700 1 $aCURCIO, B.R.
700 1 $aGASTAL, G.D.A.
700 1 $aGHENO, L.
700 1 $aJUNIOR, A.S.V.
700 1 $aCORCINI, C.D.
700 1 $aNOGUEIRA, C.E.W.
700 1 $aAGUIAR, F.L.N.
700 1 $aGASTAL, E.L.
773 $tReproductive Biology, December 2023, Volume 23, Issue 4, Article 100814. https://doi.org/10.1016/j.repbio.2023.100814
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
05/02/2020 |
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Actualizado : |
05/02/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
GONZÁLEZ, S.N.; VIEGA CAZÉRES, L.M.; BERETTA, A.; CÓRDOBA, M.A.; ROSSI, C. |
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Afiliación : |
SILVANA NOEMI GONZALEZ PARODI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS MARÍA VIEGA CAZÉRES, Universidad de la República, Facultad de Agronomía, Montevideo, Uruguay; ANDRES NICOLAS BERETTA BLANCO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIANO AUGUSTO CÓRDOBA, Universidad Nacional de Córdoba, Facultad de Ciencias Agropecuarias, Ciudad Universitaria, Córdoba, Argentina; CARLOS ALBERTO ROSSI RODRIGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Efecto de la temperatura y la precipitación durante el llenado de grano sobre la dormición y sensibilidad al agua en granos de cebada (Hordeum vulgare L.). [Effect of temperature and precipitation during grain filling on dormancy and water sensitivity in barley grains]. |
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Fecha de publicación : |
2019 |
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Fuente / Imprenta : |
AgriScientia, 2019, Volume 36, Issue 2, Pages 19-27. OPEN ACCESS. Doi: https://doi.org/10.31047/1668.298x.v36.n2.21867 |
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ISSN : |
0327-6244 |
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DOI : |
10.31047/1668.298x.v36.n2.21867 |
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Idioma : |
Español |
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Notas : |
Article history: Recibido: 26 Octubre 2018 / Aceptado: 18 Diciembre 2019.
Corresponding author: Silvana González Parodi (sngonzalez@inia.org.uy) |
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Contenido : |
ABSTRACT.
After harvesting, barley grains (Hordeum vulgare L.) can show dormancy and water sensitivity, which delays industrial use. Both effects depend on the barley genotype and environmental conditions during grain maturation. In order to analyze the effect of the average temperature and the total rainfall accumulated during and in the final third of the grain filling, on the dormancy and water sensitivity and the relationship between them, three cultivars of barley were sownin four dates in 2009 and two in 2011. Temperature and daily rainfall were recorded from anthesis to physiological maturity. The dormancy was evaluated as germination at 12 days post physiological maturity and the water sensitivity as germination in water excess conditions at 100 days post physiological maturity. The variables that presented a higher correlation with germination and water sensitivity were average temperature and accumulated rainfall during grain maturity. Environments with average high temperature during grain filling, reduced dormancy, but on water sensitivity decrease was cultivar dependent. The increase of rainfall during grain filling, decreased dormancy, but increased water sensitivity in all three cultivars. © 2019, Universidad Nacional de Cordoba. All rights reserved.
RESUMEN.
Los granos de cebada (Hordeum vulgare L.) pueden presentar dormición y sensibilidad al agua poscosecha, que retrasan su utilización industrial. Ambas, dependen del genotipo y del ambiente durante la maduración del grano. Con el fin de analizar el efecto de la temperatura media y las precipitaciones acumuladas durante y en el tercio final del llenado de grano, sobre la dormición y la sensibilidad al agua y la relación entre ellas, fueron sembrados tres cultivares de cebada en cuatro fechas durante el 2009 y dos durante el 2011. Se registraron temperaturas y precipitaciones diarias desde antesis a madurez fisiológica. La dormición se evaluó como la germinación a los 12 días pos-madurez fisiológica y la sensibilidad al agua como la germinación en exceso de agua, a los 100 días pos-madurez fisiológica. Las variables que presentaron mejor correlación con germinación y sensibilidad al agua fueron la temperatura media y las precipitaciones acumuladas durante el llenado de grano. Ambientes con mayores temperaturas medias durante el llenado de grano disminuyeron la dormición, pero la disminución de la sensibilidad al agua dependió del cultivar. El incremento de las precipitaciones durante el llenado de grano disminuyó la dormición, pero aumentó la sensibilidad al agua en los tres cultivares MenosABSTRACT.
After harvesting, barley grains (Hordeum vulgare L.) can show dormancy and water sensitivity, which delays industrial use. Both effects depend on the barley genotype and environmental conditions during grain maturation. In order to analyze the effect of the average temperature and the total rainfall accumulated during and in the final third of the grain filling, on the dormancy and water sensitivity and the relationship between them, three cultivars of barley were sownin four dates in 2009 and two in 2011. Temperature and daily rainfall were recorded from anthesis to physiological maturity. The dormancy was evaluated as germination at 12 days post physiological maturity and the water sensitivity as germination in water excess conditions at 100 days post physiological maturity. The variables that presented a higher correlation with germination and water sensitivity were average temperature and accumulated rainfall during grain maturity. Environments with average high temperature during grain filling, reduced dormancy, but on water sensitivity decrease was cultivar dependent. The increase of rainfall during grain filling, decreased dormancy, but increased water sensitivity in all three cultivars. © 2019, Universidad Nacional de Cordoba. All rights reserved.
RESUMEN.
Los granos de cebada (Hordeum vulgare L.) pueden presentar dormición y sensibilidad al agua poscosecha, que retrasan su utilización industrial. Ambas, dependen del genotipo y del ambiente... Presentar Todo |
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Palabras claves : |
Efectos ambientales. |
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Thesagro : |
ANALISIS; GERMINACION; HORDEUM VULGARE; MALTA. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/14126/1/21867-Article-Text-80629-1-10-20191224.pdf
https://revistas.unc.edu.ar/index.php/agris/article/view/21867/28849
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Marc : |
LEADER 03800naa a2200265 a 4500
001 1060765
005 2020-02-05
008 2019 bl uuuu u00u1 u #d
022 $a0327-6244
024 7 $a10.31047/1668.298x.v36.n2.21867$2DOI
100 1 $aGONZÁLEZ, S.N.
245 $aEfecto de la temperatura y la precipitación durante el llenado de grano sobre la dormición y sensibilidad al agua en granos de cebada (Hordeum vulgare L.). [Effect of temperature and precipitation during grain filling on dormancy and water sensitivity in barley grains].$h[electronic resource]
260 $c2019
500 $aArticle history: Recibido: 26 Octubre 2018 / Aceptado: 18 Diciembre 2019. Corresponding author: Silvana González Parodi (sngonzalez@inia.org.uy)
520 $aABSTRACT. After harvesting, barley grains (Hordeum vulgare L.) can show dormancy and water sensitivity, which delays industrial use. Both effects depend on the barley genotype and environmental conditions during grain maturation. In order to analyze the effect of the average temperature and the total rainfall accumulated during and in the final third of the grain filling, on the dormancy and water sensitivity and the relationship between them, three cultivars of barley were sownin four dates in 2009 and two in 2011. Temperature and daily rainfall were recorded from anthesis to physiological maturity. The dormancy was evaluated as germination at 12 days post physiological maturity and the water sensitivity as germination in water excess conditions at 100 days post physiological maturity. The variables that presented a higher correlation with germination and water sensitivity were average temperature and accumulated rainfall during grain maturity. Environments with average high temperature during grain filling, reduced dormancy, but on water sensitivity decrease was cultivar dependent. The increase of rainfall during grain filling, decreased dormancy, but increased water sensitivity in all three cultivars. © 2019, Universidad Nacional de Cordoba. All rights reserved. RESUMEN. Los granos de cebada (Hordeum vulgare L.) pueden presentar dormición y sensibilidad al agua poscosecha, que retrasan su utilización industrial. Ambas, dependen del genotipo y del ambiente durante la maduración del grano. Con el fin de analizar el efecto de la temperatura media y las precipitaciones acumuladas durante y en el tercio final del llenado de grano, sobre la dormición y la sensibilidad al agua y la relación entre ellas, fueron sembrados tres cultivares de cebada en cuatro fechas durante el 2009 y dos durante el 2011. Se registraron temperaturas y precipitaciones diarias desde antesis a madurez fisiológica. La dormición se evaluó como la germinación a los 12 días pos-madurez fisiológica y la sensibilidad al agua como la germinación en exceso de agua, a los 100 días pos-madurez fisiológica. Las variables que presentaron mejor correlación con germinación y sensibilidad al agua fueron la temperatura media y las precipitaciones acumuladas durante el llenado de grano. Ambientes con mayores temperaturas medias durante el llenado de grano disminuyeron la dormición, pero la disminución de la sensibilidad al agua dependió del cultivar. El incremento de las precipitaciones durante el llenado de grano disminuyó la dormición, pero aumentó la sensibilidad al agua en los tres cultivares
650 $aANALISIS
650 $aGERMINACION
650 $aHORDEUM VULGARE
650 $aMALTA
653 $aEfectos ambientales
700 1 $aVIEGA CAZÉRES, L.M.
700 1 $aBERETTA, A.
700 1 $aCÓRDOBA, M.A.
700 1 $aROSSI, C.
773 $tAgriScientia, 2019, Volume 36, Issue 2, Pages 19-27. OPEN ACCESS. Doi: https://doi.org/10.31047/1668.298x.v36.n2.21867
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